Regulatory

Part:BBa_K3425101:Design

Designed by: Rubén Crespo Blanco   Group: iGEM20_UofUppsala   (2020-10-08)


pCadBA operator and promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Constitutive expression of BBa_K3425100 has leaky expression, but dimerization increases the expression of downstream translational units notably. Native CadC may induce expression too, but we didn't found evidence of this on LB or other typically used growth media.


Source

Rhee, J. E., Kim, K. S., and Choi, S. H. (2005). CadC activates pH-dependent expression of the Vibrio vulnificus cadBA operon at a distance through direct binding to an upstream region. Journal of bacteriology, 187(22), 7870-7875.

References